| - | ITA Regional Champ |
| 128 | Sydney Marin (GCW) def. Olivia Herzog (UWEC) 7-6, 6-5 |
| 128 | Tan Mukherjee (GCW) def. Reese Burton (UWEC) 6-3, 6-1 |
| 128 | Kalea Martin (GCW) def. Sydney Wiener (UW-RF) 6-1, 6-0 |
| 128 | Anika Sheth (GCW) def. Emma Thole (BETHEL) 6-4, 7-5 |
| 64 | Lily Perrin (GCW) def. Giovanna Mendes (CARTH) 7-5, 6-2 |
| 64 | Molly Austin (GUSTAV) def. Sydney Marin (GCW) 6-2, 6-2 |
| 64 | Paige Sargent (GCW) def. Mac Graves (CORNELL) 6-1, 6-1 |
| 64 | Tan Mukherjee (GCW) def. Allison Szalay (GUSTAV) 4-6, 6-3, 10-8 |
| 64 | Pari Chavan (GCW) def. Fiona O'Flaherty (STOLAF) 6-3, 6-1 |
| 64 | Zoe Rose (CARLE) def. Kalea Martin (GCW) 6-4, 6-4 |
| 64 | Utsah Kalra (GCW) def. Julia Baber (BETHEL) 6-2, 7-5 |
| 64 | Miriam Shteyman (CARLE) def. Anika Sheth (GCW) 6-4, 7-5 |
| 64 | Erin Jarvis (GCW) def. Ella De Young (GUSTAV) 3-6, 6-3, 10-4 |
| 32 | Kaya De Bruijn (GUSTAV) def. Lily Perrin (GCW) 6-1, 6-2 |
| 32 | Claire Keller (BETHEL) def. Paige Sargent (GCW) 6-4, 6-4 |
| 32 | Kelsey Pickens (CARLE) def. Tan Mukherjee (GCW) 6-4, 6-3 |
| 32 | Pari Chavan (GCW) def. Sophia Fain (COE) 6-0, 7-5 |
| 32 | Karina Elvestrom (GUSTAV) def. Utsah Kalra (GCW) 6-2, 7-6 |
| 32 | Lauryn Douglas (BETHEL) def. Erin Jarvis (GCW) 6-4, 6-1 |
| 16 | Pari Chavan (GCW) def. Sam Fuchs (UWEC) 3-6, 6-3, 10-3 |
| QF | Karina Elvestrom (GUSTAV) def. Pari Chavan (GCW) 4-6, 6-4, 6-3 |
| - | Midwest Invite |
| 1-16 | Keira Healy (GCW) def. Sophie Lipa (GUSTAV) 6-0, 6-0 |
| 2-16 | Annalise Skrade (CON-MOOR) def. Addie Persian (GCW) 1-6, 7-5, 15-13 |
| 3-16 | Nehir Ergun (GCW) def. Sarah Baker (UW-RF) 6-4, 7-5 |
| 4-16 | Alexa Viersma (GCW) def. Izzy Sullivan (NW-SP) 6-2, 6-1 |
| 5-16 | Tea Armstrong (UWEC) def. Alyssa Trapp (GCW) 6-1, 10-8 |
| 8-16 | Nina Perkins (GCW) def. Lila Collins (STBEN) 4-6, 7-5, 10-8 |
| 9-16 | Sofia Bolanos (GCW) def. Jessica Kukowski (UW-SUP) 6-2, 6-3 |
| 1-QF | Keira Healy (GCW) def. Emily Eisma (BETHEL) 6-1, 6-1 |
| 3-QF | Nehir Ergun (GCW) def. Allyson Yahn (STBEN) 6-2, 6-2 |
| 4-QF | Alexa Viersma (GCW) def. Anna Paula Ricaute (UWEC) 6-0, 6-4 |
| 8-QF | Nina Perkins (GCW) def. Annie Klemmensen (GUSTAV) 4-6, 6-0, 10-3 |
| 9-QF | Becca Vala (CENTRAL) def. Sofia Bolanos (GCW) 6-7, 6-4, 10-6 |
| 1-SF | Keira Healy (GCW) def. Emma Thurness (WART) 6-1, 3-6, 11-9 |
| 3-SF | Nehir Ergun (GCW) def. Sarah Clark (STOLAF) 6-1, 6-0 |
| 4-SF | Victoria Rizzo (CARTH) def. Alexa Viersma (GCW) 3-3, retired |
| 8-SF | Nina Perkins (GCW) def. Caroline Hutchinson (LORAS) 6-2, 6-1 |
| 1-1ST | Keira Healy (GCW) def. Aubrey Whittwer (UWEC) 3-6, 7-5, 11-9 |
| 3-1ST | Nehir Ergun (GCW) def. Elif Oezen (UW-W) 6-2, 6-3 |
| 8-1ST | Nina Perkins (GCW) def. Katie Thompson (GUSTAV) 6-4, 6-3 |
| - | Consolation Singles |
| 3-16 | Sofia Bolanos (GCW) def. Hannah Byhre (UWEC) 8-3 |
| 3-QF | Sofia Bolanos (GCW) def. Sailor Mohlenbrock (BETHLUTH) by default |
| 3-SF | Sofia Bolanos (GCW) def. Lena Lichau (LUTHER) 8-3 |
| 3-1ST | Malia McKinnon (BETHEL) def. Sofia Bolanos (GCW) 8-2 |
